Description
General description
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Application
HEPES has been used:
In the novel vesicle-budding protocol as a component in stock solution and buffer solutions
As a component in intracellular solution for the preparation of brain-slice recording
As a component of wash buffer, elution buffer and desalting buffer during affinity protein purification
To maintain the pH at 7 in thermal denaturation assay of purified protein
In isocratic elution of protein using gel filtration
Along with NaCl in sample preparation for EPR (Electron Paramagnetic Resonance) spectroscopy
Legal Information
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