Merck, S-014-B, Enzyme Free Cell Dissociation Solution PBS Based (1X), liquid, 500ml

General description
Enzyme-Free Cell Dissociation Solution is a PBS based proprietary formulation of chelating agents and agents to stabilize their activity on cells. The enzymatic activity of this formulation is comparable to trypsin. It preserves the structural and functional integrity of cell surface proteins and does not have the cytotoxic effects sometimes associated with 0.5 mM EDTA. It can be used to dissociate primary cells, tissues, and tumors with an increased plating efficiency.

Enzyme-Free dissociation solution should be used with cells that are sub-confluent (60-80%). If cells are more than 80% confluent, we recommend trypsinizing the cells and replating at 30-40% confluency 24 hours before enzyme-free use (this will allow the cells to be 60-80% confluent for enzyme free dissociation).
Preparation Note
Product can be stored at room temperature or at 4°C. Shelf life is 2 years
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.