Merck, S0902-1L, SSC Buffer

General description
SSC Buffer 20x Powder is a standard reagent in Southern and Northern blotting procedures. This concentrated powder should be reconstituted in molecular biology grade water to the indicated final volume before use (final solution is a 20x). The 20x concentrate can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5x.
Application
Saline-sodium citrate (SSC) Buffer has been used as a washing buffer in microarray technique. It has also been used as a constituent of fluorescence in situ hybridization (FISH) buffer.
Suitable for:
Preparing nucleic acids for hybridization
Hybridization buffer for Northern and Southern blots
Washing buffer for Northern and Southern blots

Features and Benefits
Multi-purpose use in hybridizations
Easily reconstituted and diluted to any concentration needed

Preparation Note
Final SSC Buffer 20x Concentrate contains 0.3 M sodium citrate in 3M NaCl.