Merck, S1629-10UN, Sulfatase from Aerobacter aerogenes

General description
Sulfatases comprise Cys/Ser-X-Pro-X-Arg motif that is conserved in their active sites.
Application
Sulfatase from Aerobacter aerogenes has been used:
as a deconjugation enzyme for treating plasma samples for quercetin quantification using liquid chromatography with tandem mass spectrometry (LC/MS/MS) analyses
in fluorescence intensity-based enzymatic assay with an activity-based probe probe 1
to treat sulfatide liposomes to remove the 3-O-sulfogalactosyde head from sulfatides

Biochem/physiol Actions
Sulfatases hydrolyze sulfate ester bonds to generate inorganic sulfates. Microbial sulfatases participate in sulfur scavenging and are essential enzymes for the utilization of sulfur. They may be associated with pathogenesis. Commercially available sulfatase from Aerobacter aerogenes is useful as a deconjugation enzyme for the removal of glucuronate and sulfate moieties from conjugates. Sulfatases find application in industry and agriculture.
Physical form
Solution in 50% glycerol containing 0.01 M Tris, pH 7.5.
Other Notes
One unit will hydrolyze 1.0 μmole of p-nitrophenyl sulfate per min at pH 7.1 at 37 °C.