Description
General description
Goat Anti-Mouse IgG1(Fcγ) Biotin Alpaca Heavy chain only Antibody is designed to specifically detect mouse IgG1 Fcγ fragment. It is a monoclonal, recombinant, goat IgG Fc fused single domain antibody to mouse IgG1 Fcγ fragment coupled to Biotin. Based on immunoelectrophoresis and/or ELISA, AGoat Anti-Mouse IgG1(Fcγ) Biotin Alpaca Heavy chain only Antibody reacts with the Fc fragment of mouse IgG1 selectively but not with other mouse IgG subclasses, mouse IgM, or the Fab portion of mouse immunoglobulins.
Most monoclonal antibodies are generated in mouse. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mouse. Each isotype has a different heavy chain. Mouse IgG constitutes 75% of serum immunoglobulins, and IgG is the predominant form of first antibody produced from mouse. Mouse IgG consists of five subclasses-IgG1, IgG2a, IgG2b, IgG2c(inbred mouse strains with the Igh1-b allele have IgG2c isotype instead of IgG2a), IgG3. They are highly homologous and differ mainly in the hinge region. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains, and the heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(less than15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Immunogen
Recombinant Alpaca single variable domain secondary nano antibody
Biochem/physiol Actions
Mouse IgG1(Fcγ fragment specific)
Isotype: VHH domain of alpaca IgG2b/2c fused to goat IgG Fc
Packaging
100 μg in Glass bottle
Physical form
Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose
Preparation Note
Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL
After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Stable for 12 months at -20°C
Other Notes
It has weak cross reaction with mouse IgG3. No cross-reactivity with mouse IgG2b/2c, mouse IgM, rabbit, human, cynomolgus, rat, goat IgG
