Description
General description
Research area: Cell signaling This product is recombinant human PKM2 (P14618) expressed in E. coli and has a predicted molecular mass of 58 kDa. The embryonic pyruvate kinase M2 (PKM2) is one of the isoforms of pyruvate kinases (PK), found in mammals. It is coded by the PKM2 gene mapped to human chromosome 15q23. PKM2 is expressed in all cells excluding adult muscle, brain, and liver. This protein is usually present, as an active tetrameric form.
Application
Human pyruvate kinase M2 (PKM2) has been used to study the effects of phosphorylated PKM2 in inactivating myofibroblasts during renal fibrosis.PyruvateKinase M2 human has been used as an enzyme to study the inhibitory effect of a novel irreversible inhibitor on the kinaseactivity of PKM2. It has also been used in recombinant protein labelingand in pyruvate kinase enzyme activity assay.
Biochem/physiol Actions
Pyruvate kinase (PK) is an important glycolytic enzyme catalyzing the last step of glycolysis, transferring a phosphate group from phosphoenolpyruvate to ADP to yield one molecule of ATP and one molecule of pyruvate. The embryonic pyruvate kinase M2 (PKM2) controls β-catenin transactivation. It plays an important role in aerobic glycolysis or the Warburg effect. PKM2 induces gene transcription and tumorigenesis.Pyruvate Kinase M2 (PKM2)plays a role in the regulation of cell growth, cell proliferation, andapoptosis. It is found predominantly in embryonic tissues and several humancancers. PKM2 acts as a cytosolic thyroid hormone binding protein(CTHBP) and as a phosphotyrosine binding protein. PKM2 binds tophosphotyrosine peptides which results in the release of its allostericactivator fructose-1,6-bisphosphate (FBP) is associated with the blockageof PKM2 activity.
Physical form
Supplied as a lyophilized powder containing phosphate buffer at pH7.5, NaCl, DTT and a carbohydrate stabilizer.
Other Notes
One unit will convert 1.0 μmole of phosphoenol-pyruvate to pyruvate per minute at pH 7.6 at 37 °C, in the presence of 1 mM fructose-1,6-bisphosphate.
