General description
The YUMM (Yale University Mouse Melanoma) cell lines were developed as models that recapitulate genetic drivers found in a significant proportion of human melanomas. YUMM5.4 cells are syngeneic with the immunocompetent C57Bl/6 mouse model.
Immunocompetent genetically-engineered mouse models with select genetic mutations that drive melanoma are fundamental for studying candidate immunotherapies, but are limited by the need to maintain colonies of multiple genotypes necessary to generate models with appropriate genetic backgrounds.Approximately half of all melanomas harbor Braf mutations, of which the V600E mutation comprises 90% of cases, while the tumor suppressor p53 is inactivated in 90% of melanomas. The YUMM5.4 cell line harbors the activating Braf V600E mutation and is negative for wild-type p53.SourceYUMM5.4 mouse melanoma cell line was derived from a 4-hydroxytamoxifen-induced melanoma tumor in a male C57BL/6 mouse into which mutations from the Braf/Pten genetically engineered mouse model were introduced by backcrossing.References:1. Meeth K et al. (2016) The YUMM lines: a series of congenic mouse melanoma cell lines with defined genetic alterations. Pigment Cell Melanoma Res 29(5): 590-597.2. Dankort D et al. (2009) Braf(V600E) cooperates with Pten loss to induce metastatic melanoma. Nat Genet. 41(5): 544-552.
Application
Each vial contains > 1X106 viable cells.
YUMM5.4 cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
Cells are verified to be of mouse origin and negative for interspecies contamination from rat, chinese hamster, Golden Syrian hamster, human and nonhuman primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
Cells are negative for mycoplasma contamination."
Features and Benefits
The YUMM cell lines recapitulate genetic drivers found in many human melanomas. YUMM5.4 retains WT Pten, permitting comparative analysis for parsing distinct contributions of PI3K-Akt signaling in melanoma tumorigenesis and immune evasion.
Preparation Note
YUMM5.4 cells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Other Notes
Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact
[email protected].
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
