Application
721.221 Human HLA negative B-lymphoblastoid cells are verified to be of human origin and negative for mouse, rat, Chinese hamster, Golden Syrian hamster, and non-human primate interspecies contamination, as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
Cells tested negative for infectious diseases against a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
Cells tested negative for mycoplasma.
The human leukocyte antigen (HLA) gene complex encodes the major histocompatibility complex (MHC) proteins, key regulators of the human immune system. MHC proteins carry antigenic determinants for cell-surface recognition by T cells, and allow for immune autorecognition.1 The diversity of antigens provides a challenge for parsing individual T-cell activators and specific immune responses, and the ability to manipulate specific determinants is a paramount factor for understanding autoimmune diseases that arise from MHC dysfunction.
The 721.221 human HLA-negative B cell lymphoblastoid cell line is a well-established model for immune activation and HLA expression. The absence of class I HLA expression in 721.221 cells allows for functional analysis of individual class I antigens via transformation with individual HLA genes.2 HLA genes are ectopically expressed at quantitatively normal levels in 721.21 cells.2 721.221 cells express the B-cell marker CD19 4 and several NK activator ligands including CD48, CD80, and CD86. 5 The unique characteristics of the 721.221 cell line make it a highly versatile system for immunology research.
Source
The 721.221 cell line was derived from 721 lymphoblastoid cell line exposed to gamma radiation, inducing null mutations in endogenous HLA-A, B, and C class I antigen genes.
References
1. Simpson E. Immunol Suppl. 1988; 1:27-30.
2. Shimizu Y, DeMars R. J Immunol. 1989; 142(9):3320-3328.
3. Lisovsky I, Isitman G, Bruneau J, Bernard NF. J Leukoc Biol 2015; 97(4):761-767.
4. DeBell KE, Simhadri VR, Mariano JL, Borrego F. BMC Immunol 2012; 13:23.
5. Tremblay-McLean A, Coenraads S, Kiani Z, Dupuy FP, Bernard NF. BMC Immunol 2019; 20(1):8
Features and Benefits
The unique characteristics of the 721.221 cell line make it a highly versatile system for immunology research.
Preparation Note
Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Other Notes
Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact
[email protected].
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
