ApplicationGonadotropin-releasing hormone (GnRH) plays a key role in the control of reproduction in mammals. GnRH acts via its receptor, GnRH-R, to trigger the synthesis and release of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) by the pituitary gonadotropes. In turn, the gonadotropins regulate gametogenesis and steroidogenesis in the gonads. LH and FSH comprise a common glycoprotein hormone subunit, CGA, and the respective specific subunits LH and FSH. The individual subunits bind noncovalently to the common a subunit to form the mature glycoprotein hormones. Lhb gene expression is preferentially induced by high-frequency GnRH pulses, whereas low-frequency pulses favor Fshb expression.
Source.
The immortalized L(betta)T2 mouse pituitary gonadotrope cell line was generated by targeted tumorigenesis in transgenic mice carrying the rat LHb regulatory region linked to the SV40 T-antigen oncogene.1 L(betta)T2 cells express CGA, GnRH-R, and LHb. The cell line responds to pulsatile GnRH stimulation by upregulating Lhb and Gnrhr and secreting LH. The L(betta)T2 cell line has been widely used as an in vitro model for the study of gonadotropin gene regulation and GnRH signaling.2,3,4,5 The global mRNA expression profile and a genome-wide atlas of accessible chromatin in GnRH-stimulated L(betta)T2 cells has recently been described6 and the STR profile has also been reported.7
References
Note: Please include the appropriate references from 1-5 in your publications that utilize them.
1. Alarid ET, Windle JJ, Whyte DB, Mellon PL. Development 1996; 122(10):3319-3329.
2. Turgeon JL, Kimura Y, Waring DW, Mellon PL. Molecular Endocrinology 1996; 10(4):439-450.
3. Thomas P, Mellon PL, Turgeon J, Waring DW. Endocrinology 1996; 137(7):2929-2989.
4. Alarid ET, Holley S, Hayakawa M, Mellon PL. Mol Cell Endocrinol 1998; 140(1-2):25-30.
5. Pernasetti F, Vasilyev VV, Rosenberg SB, Bailey JS, Huang HJ, Miller WL, Mellon PL. Endocrinology 2001; 142(6):2284-2295.
6. Ruf-Zamojski F et al. Front Endocrinol (Lausanne) 2018; 9:34.
7. Ruf-Zamojski F et al. J Endocr Soc 2019; 3(5):902–920.
Features and BenefitsThe LbetaT2 cell line has been widely used as an in vitro model for the study of gonadotropin gene regulation and GnRH signaling
Preparation NoteThe cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Other NotesSubject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact
[email protected].
DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
