General description
Colorectal cancer accounts for roughly 10% of all cancer cases worldwide with more than half of all patients with CRC developing metastatic disease leading to death. Recently, tissue derived organoids have emerged as a more predictive 3 dimensional cell culture model of disease. 3D organoid cultures conserve the original genetic and phenotypic characteristics of the primary tissue allowing for their application in many research fields included drug development, personalized medicine and potential therapeutics. In vitro cultured tumor organoids have also been shown to predict patient response to chemotherapeutics. PDOs derived from colorectal cancer (CRC organoids) have been used for cell modeling and to investigate the function of cancer related driver gene mutations including APC, TP53, KRAS, BRAF, PIK3CA etc.
We are now offering a comprehensive biobank of highly characterized tissue-derived human gastrointestinal organoids from normal and diseased patients. The intestinal organoid biobank contains over 50 highly characterized intestinal organoids from both normal and diseased patients derived from multiple regions of the digestive systems including small intestine (duodenum, ileum), stomach, rectum and colon.
Cell Line Characteristics
Sex: Male
Age: 74 Years
Organ: Colon (Xenograph from Primary Tumor)
Disease: Adenocarcinoma (KRAS Mutation: G12A)
Application
Viability: >=1500 viable organoids/vial
Organoid Growth: Pass
Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
Mycoplasma Contamination: Negative
STR Profile: Pass
Features and Benefits
Cryopreserved patient derived colorectal cancer organoids (PDOs)
Preparation Note
Store in liquid nitrogen. The organoids can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Legal Information
3dGRO® is a trademark of Merck KGaA, Darmstadt, Germany.
3dGRO® organoids were derived utilizing HUB Organoid Technology.
Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/pdouse. This product may not be re-engineered or copied; used to make derivatives or modifications; used to develop, test, or manufacture a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact
[email protected].
3dGRO is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
