Description
General description
EMD Millipore’s Dopaminergic Differentiation Growth Factor Sampler contains a collection of five validated growth factors that are routinely used to induce differentiation of human pluripotent ES/iPS cells to dopaminergic neurons along with a reliable high affinity antibody marker, anti-tyrosine hydrodylase, to aid in quantifying the percentage yields from differentiation experiments.
Product Source: E. coli.
Application
Research Category
Stem Cell Research
Research Sub Category
Growth Factors & Receptors
This Dopaminergic Differentiation Growth Factor Sampler contains five validated growth factors used to induce differentiation of human pluripotent Embryonic stem (ES) & induced pluripotent Stem (iPS) cells to dopaminergic neurons.
Biochem/physiol Actions
Cross Reactivty
None
Preparation Note
Store at -20°C for up to 6 months from date of receipt.
Analysis Note
Using the growth factors supplied in this kit a 3-5 fold increase of dopaminergic differention (up to 50-70% TH+ cells) was routinely produced using HES/hips cell derived neural progenitor cells vs. spontaneous neuronal differentiation.
Other Notes
1. Sonic HedgeHog (Shh), Human Recombinant (Part No. GF174-5UG): Two 5 μg vials. Lyophilized powder. Store at -20°C.
2. Fibroblast Growth Factor-8 (FGF-8), Human Recombinant (Part No. GF110-5UG): One 5 μg vial. Lyophilized powder. Store at -20°C.
3. Brain Derived Neurotrophic Factor (BDNF), Human Recombinant (Part No. GF029-2UG): One 2 μg vial. Lyophilized powder. Store at -20°C.
4. Glial Derived Neurotrophic Factor (GDNF), Human Recombinant (Part No. GF030-2UG): One 2 μg vial. Lyophilized powder. Store at -20°C.
5. Transforming Growth Factor β-III (TGF-β-III), Human Recombinant (Part No. GF176-2UG): One 2 μg vial. Lyophilized powder. Store at -20°C.
6. Anti-Tyrosine Hydroxylase antibody, rabbit polyclonal (Part No. AB152-20): One 20 μL vial. Store at -20°C.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
