Description
General description
Lyticase is an enzyme obtained from Arthrobacter Luteus that can extract DNA from yeast by inducing partial spheroplast formation which are later lysed to release DNA. It is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation. Lyticase contains β-(1→3)-glucan laminaripentaohydrolase with additional β-(1→3)-glucanase, protease, and mannanase activities.
Application
For isolation of nucleic acids, lyticase is used in the lysis of yeast cell walls (e.g. Candida, Debaryomyces, Saccharomyces, Saccharomycopsis, Saccharomycodes, Eremothecium, and Schwanniomyces species).
Biochem/physiol Actions
Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan.
Features and Benefits
Features: Qualified Raw Materials: Products that meet the stringent requirements of the Elevated Assurance Program, ensuring compliance with regulatory standards.
Comprehensive Documentation: Includes essential documents such as certificates, impurity profiles, and declarations to support audits and risk assessments.
M-Clarity™ Quality Attributes: Products are classified within the MQ300 quality segment or higher, demonstrating superior quality assurance. M-Clarity™ Program
Confirmed Process Controls: Established processes that ensure consistency and reliability in manufacturing.
Enhanced Change Notification: Timely updates on any changes to product specifications or processes, ensuring transparency and compliance.
Freely Available Dossiers: Access to robust, ready-to-present product dossiers that include: Site quality assessment
ISO 9001 certificate
Detailed product specifications
Relevant declarations/statements (e.g., animal origin content, BSE/TSE, nitrosamine)
Benefits: Regulatory Compliance: Helps you meet compliance standards effectively, reducing the risk of regulatory issues.
Audit Readiness: Instills confidence during audits with comprehensive documentation and confirmed process controls, demonstrating your commitment to high-quality products.
Operational Transparency: Supports supply chain transparency, allowing you to track and verify the quality of raw materials.
Enhanced Support: Provides exceptional quality-related information and tailored assistance to meet your specific needs.
Risk Mitigation: Facilitates thorough risk assessments with detailed documentation, helping you manage potential compliance challenges.
Informed Decision-Making: Access to clear and comprehensive product information enables better decision-making and enhances operational efficiency.
Other Notes
One unit will produce a ΔA₈₀₀ of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Legal Information
M-CLARITY is a trademark of Merck KGaA, Darmstadt, Germany
