Description
General description
Proteinase K is an extracellular endopeptidase. It is synthesized by the mold Tritirachium album Limber. Proteinase K belongs to a new subfamily of the subtilisins. It is a 277 amino acid protein with a molecular weight of 28,930 Da. Active proteinase K is characterized with an unhydrolysed protein chain and autolysed polypeptide chains.
Application
Proteinase K catalyzes the hydrolysis of keratin.
Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.
Biochem/physiol Actions
Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp³⁹-His⁶⁹-Ser²²⁴). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.
Proteinase K catalyzes the hydrolysis of keratin.
Features and Benefits
Features: Qualified Raw Materials: Products that meet the stringent requirements of the Elevated Assurance Program, ensuring compliance with regulatory standards.
Comprehensive Documentation: Includes essential documents such as certificates, impurity profiles, and declarations to support audits and risk assessments.
M-Clarity™ Quality Attributes: Products are classified within the MQ300 quality segment or higher, demonstrating superior quality assurance. M-Clarity™ Program
Confirmed Process Controls: Established processes that ensure consistency and reliability in manufacturing.
Enhanced Change Notification: Timely updates on any changes to product specifications or processes, ensuring transparency and compliance.
Freely Available Dossiers: Access to robust, ready-to-present product dossiers that include: Site quality assessment
ISO 9001 certificate
Detailed product specifications
Relevant declarations/statements (e.g., animal origin content, BSE/TSE, nitrosamine)
Benefits: Regulatory Compliance: Helps you meet compliance standards effectively, reducing the risk of regulatory issues.
Audit Readiness: Instills confidence during audits with comprehensive documentation and confirmed process controls, demonstrating your commitment to high-quality products.
Operational Transparency: Supports supply chain transparency, allowing you to track and verify the quality of raw materials.
Enhanced Support: Provides exceptional quality-related information and tailored assistance to meet your specific needs.
Risk Mitigation: Facilitates thorough risk assessments with detailed documentation, helping you manage potential compliance challenges.
Informed Decision-Making: Access to clear and comprehensive product information enables better decision-making and enhances operational efficiency.
Other Notes
One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
Legal Information
M-CLARITY is a trademark of Merck KGaA, Darmstadt, Germany
