Merck, T0251-25MG, Thymidine 5′-triphosphate sodium salt

Application
Thymidine 5′-triphosphate (dTTP) is one of the four natural deoxynucleotides, along with deoxyadenosine 5′-triphosphate (dATP), deoxyguanosine 5′-triphosphate (dGTP) and deoxycytosine 5′-triphosphate (dCTP) used for the biosynthesis of deoxyribonucleic acids by DNA polymerases and reverse transcriptases. Deoxy-TTP may also be used as a substrate to study the specificity and kinetics of thymidine-triphosphatase(s) (EC 3.6.1.39) and other dTTP hydrolyzing activities.
Thymidine 5′-triphosphate sodium salt has been used:
in the quenching ability test for dual-aptamer and multiple-nucleotide detection based on a multiple-aptamer/GO-nS complex
to study protein-metabolite interactions in the central metabolism of Escherichia coli
in a polymerase chain reaction (PCR) product formation using AM-toxin primers

Biochem/physiol Actions
Thymidine triphosphate is a building block of DNA. It acts as an allosteric regulator of other nucleotides′ metabolism.