Merck, T1378-500G, Sigma 7-9®

General description
Sigma 7-9® also known as Trizma base or tris(hydroxymethyl)aminomethane, is a versatile biological buffer used in various applications, such as protein freezing, electrophoresis, and the preparation of buffer solutions such as TAE and TBE. With a pKa of 8.06, Tris is particularly valuable in labs working within the typical physiological pH range of living organisms (pH 7.0-9.0). It is also commonly employed in combination with other buffering agents to precisely adjust solution pH.

Additionally, Tris aids in enhancing the permeability of cell membranes. This compound is indispensable in laboratories where it is utilized to prepare buffers compatible with biological fluids, serve as a standard pH solution, and facilitate procedures such as lactate dehydrogenase assays, in situ hybridization, and protein extraction from cells.
Application
Sigma 7-9® has been used:

as a buffer for sample preparation in a study involving Enzyme-linked immunosorbent assay (ELISA)
as a buffer for sample preparation in research involving immunohistochemistry and immunofluorescence

Features and Benefits
Suitable for Biological and Biochemical Research
Effective Buffering from pH 7-9 (25 °C) with a pKa of 8.1 (25 °C)

Other Notes
For additional information on our range of Biochemicals, please complete this form.
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Sigma 7-9 is a registered trademark of Merck KGaA, Darmstadt, Germany
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany