Description
General description
Tris HCl, or Trizma® hydrochloride, is a widely utilized acidic buffer in the physiological pH range of 7.3 to 7.5. With a pKa of 8.08 at 25°C, it offers a pH range of 7.0 to 9.0, aligning with living organisms′ typical pH. Essential in various biological, cell biology, and biochemistry applications, Tris HCl is crucial for effective pH control in buffer solutions. Notably, Tris base and Tris hydrochloride alone lack sufficient buffering capacity, prompting their combination within the pH range of 7 to 9.
Tris buffers play a key role in techniques like protein electrophoresis and western blotting, serving in low ionic strength buffers for protein transfer. They are also integral to SDS-PAGE gels, running buffers, and blotting buffers. Additionally, Tris salts, especially in TAE and TBE buffers, are extensively employed in DNA agarose electrophoresis. These buffers are pivotal in diverse applications, including biochemistry, protein purification, and electrophoresis, showcasing the versatility and importance of Tris HCl in maintaining optimal pH for biochemical and cell biology research.
Application
Trizma® hydrochloride has been used as a buffer solution for staining procedure, reduction and alkylation studies and in situ hybridization procedure.
Features and Benefits
Ideal as a Biological buffer for Cell Biology and Biochemical research
Effective Buffering from pH 7-9 with a pKa of 8.1 (25 °C)
Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
Packaging
The DRYPOUR®packaging system for high-quality hygroscopic salts DRYPOUR®packaging: The current DRYPOUR®packaging configuration is in a 50L nestable drum
25KG-DP are the standard prepack offering
Smaller volumes can be put in the same drum for variable configurations
The benefits of DRYPOUR®: Improves operational efficiency by reducing material preparation time and increasing operator safety
Minimizes caking of hygroscopic salts
Offers triple protection: against moisture inside and outside, and against contamination
How does the DRYPOUR®packaging system Improve your operational efficiency? This state-of-the-art design packaging system minimizes caking, thus significantly reducing the time needed for material preparation
PE drum with our new tamper-evident seal, a polyethylene liner with integrated desiccant bags and a breathable interior Tyvek®liner
Desiccant bags are firmly integrated into the polyethylene liner
Triple protection delivers two invaluable results:
Dramatically reduced caking
No contamination risk from the desiccant
Other Notes
2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
For additional information on our range of Biochemicals, please complete this form.
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
DRYPOUR is a registered trademark of Merck KGaA, Darmstadt, Germany
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
Tyvek is a registered trademark of E. I. du Pont de Nemours and Company
