Merck, T6066-1KT, Trizma® base

General description
Tris base, also known as Trometamol or Trizma base, plays a crucial role in diverse research applications as a biological buffer. Widely utilized in buffer formulations like TAE and TBE buffers, its pKa of 8.1 renders it well-suited for maintaining pH within the physiological range (pH 7.0-9.0) applicable to most living organisms. Researchers, however, need to be cautious when studying proteins, as Tris may potentially interfere with the activity of certain enzymes.

This compound can be employed independently as a buffer or as part of mixed buffer formulations, including Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, among others. Notably, Tris base is pure, essentially stable, and relatively non-hygroscopic.

In laboratory settings, Tris base proves indispensable for preparing buffers compatible with biological fluids, serving as a standard pH solution, and facilitating various procedures such as lactate dehydrogenase assays, in situ hybridization, and protein extraction from cells. Its versatility extends to applications in cell culture, biochemistry, and molecular biology labs, where it aids in studies related to cell membrane permeability and buffer preparation.
Application
Trizma has been used:
As a component in the culture of human embryonic stem (hES) cells on human and mouse feeder cells
As a component of pre-hybridization buffer in in situ hybridization
To suspend dried protein before SDS-PAGE protein characterization
As a component of lysis buffer to wash and lyse cells before immunoprecipitation
In the extraction and enrichment of membrane proteins for separation by two-dimensional polyacrylamide gel electrophoresis
In studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences, by use of anion exchange HPLC
In capillary electrochromatography and UV analysis of tocopherols and tocotrienols
as a component of Tris/base solution in a sulforhodamine B (SRB) assay

Features and Benefits
Suitable for Cell culture, Biochemical and Molecular Biology Research
Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
Effective Buffering from pH 7-9 (25 °C) with a pKa of 8.1 (25 °C)
Tested for the presence of Anionic and Cationic traces
Tested for DNase, RNase, and protease

Packaging
T6066-1KT:
Each kit contains 3 x 100G samples, each sample from a uniquely manufactured lot.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany