Merck, T8787-250ML, Triton™ X-100

General description
Triton™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.
Application
Triton™ X-100 has been used:
In immunohistochemistry for staining the Flat-mount retinas
Along with ice-cold PBS (phosphate buffered saline) in suspension of cells for cell DNA analysis and Annexin V assay
To permeabilise cells during Immunofluorescent microscopic studies
As a positive control in LDH assay to determine the cell membrane integrity
For estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedure
For the preparation of outer membrane protein exctract
As a component of extraction buffer along with tris-HCl, NaCl, CaCl₂, ZnCl₂, Brij 35 for homogenization of mice lung cells
In the treatment of tissue sections for Immunofluorescence labeling

Biochem/physiol Actions
Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.
Features and Benefits
Ideal for sensitive molecular biology research applications
Tested for DNase and RNase
Non-ionic nondenaturing detergent
Superior wetting agent and emulsifier
Highly versatile surfactant

Other Notes
2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
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Legal Information
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow