Description
General description
The transforming growth factor beta (TGFβ) system, a superfamily of cytokines as well as signaling pathways, is highly conserved throughout the animal kingdom. TGFβ functions in immune cell development, angiogenesis, wound healing and embryonic development, bone development and plays a critical role in immune response, heart disease, and cancer. In its normal state TGFβ is one of the few classes of proteins able to inhibit cell growth by halting mitosis at the G1 state, inducing cell differentiation or apoptosis. However, during oncogenesis mutations in the TGFβ signaling pathway result in tumor cell resistance to the effects of normally functioning TGFβ, causing proliferation without regulation. Initial research suggests that VE-cadherin may enhance the mutated TGFβ signaling pathway.
Application
MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Analyze TGF-β1 in multi-species serum, plasma, and cell culture supernatants.
Analytes included: TGF-β1
Note: This assay requires a sample acidification treatment using the provided reagents and protocol.
Assay Characteristics: Refer to kit protocol for assay cross-reactivity, sensitivity, precision, and accuracy.
Features and Benefits
Bulk Single Plex Format: Specifically designed for the quantitative detection of TGFβ1 using Luminex® xMAP® technology.
Includes Sample Acidification Protocol: To measure active TGFβ, samples must undergo acid treatment. This step is critical for converting latent TGFβ into its active form, ensuring accurate quantification.
Multi-Species Reactivity: Assay can detect TGFβ1 in human, mouse, rat, pig, horse, non-human primate, rabbit, guinea pig, and hamster samples.
Wide Dynamic Range: Standard curve spans 9.8–10,000 pg/mL.
Focused Biomarker Analysis: Ideal for researchers studying TGFβ1-specific pathways in immunology, oncology, wound healing, and developmental biology.
Legal Information
Luminex is a registered trademark of Luminex Corporation
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Disclaimer
For research use only. Not for use in diagnostic procedures.
Label License/Sticker for Assay Product:
By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.
