Merck, W0625-10G, Wright stain

Name: Wright stain
Brand: Merck
SKU: W0625-10G

Size:10 g, Eosin Methylene blue according to Wright, suitable for blood stain

Available Catalog Numbers

W0625-100G W0625-10G W0625-25G

Catalog Number: W0625-10G

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Category

Special Stains

Pack Size10 g

TagsClinical & Diagnostics Tissue Diagnostics Hematology Stains

CollectionsMerck Tissue Diagnostics – Universal Reagents for Histology & IHC Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Wright′s Stain solution is commercially available and is generally used in hematology. Its preparation involves heating methylene blue with sodium bicarbonate and combining the mixture with eosin. It is alkaline, hence, its concentration is continuously changing due to the progressive oxidizing action of the alkali. Therefore, Giemsa′s stain is added to preserve the azurophilic properties of polychrome dyes.
Application
Reported to be superior to May-Grunwald Stain in blood staining.
Wright′s Stain is mainly used for the staining of peripheral blood smears and bone marrow smears.

It is also used for G-banding and helps in the analysis of poorly spread metaphases.
It has been used to study the cytogenetics of human hematopoietic cells.
It has also been employed in the karyotyping of human embryonic stem cells.
It is also used in the staining of chromosomes.
It is used for the exudate analysis of white blood cell concentration.

Features and Benefits
The purified dyes in Wright′s Stain eliminate inconsistent staining and yield reproducible lot-to-lot chromogenic responses.
Preparation Note
Prepared using polychromed (oxidized) methylene blue and eosin Y.

Specifications

General

UNSPSC Code	12171500
NACRES	NA.47
Web Name Suffix Override	≤4% purity (HPLC), powder
MDL number	MFCD00082143
SMILES string	[s]5c6c(nc7c5cc(cc7)N(C)C)cc[c](c6)=[N+](C)C.Brc1c2[o+]c3c(c(c2cc(c1O)Br)c4c(cccc4)C(=O)O)cc(c(c3Br)O)Br
InChI	1S/C20H8Br4O5.C16H18N3S/c21-11-5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(22)17(26)15(24)19(10)29-18(9)14(23)16(11)25;1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13/h1-6H,(H2-,25,26,27,28);5-10H,1-4H3/q;+1/p+1
InChI key	AXIKDPDWFVPGOD-UHFFFAOYSA-O
assay	≤4% (HPLC)
form	powder
concentration	≤4% (HPLC)
technique(s)	microbe id \| staining: suitable
color	dark green
solubility	methanol: 10 mg/10 mL
suitability	suitable for blood stain
application(s)	diagnostic assay manufacturinghematologyhistology
storage temp.	room temp
Quality Level	200

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