Merck, X114-5GA, Triton™ X-114

General description
Triton™ X-114 is a nonionic detergent. It is a good detergent for solubilizing membranes having reversible phase-separation property useful for the separation of intrinsic membrane proteins. Solution of Triton™ X-114 is homogeneous at 0°C but phase separation into aqueous phase and a detergent phase occurs above 20°C. Phase separation of proteins in solutions of Triton™ X-114 has been investigated. This octylphenol ethoxylate is compatible with anionic, cationic, and other nonionic surfactants and chemically stable in most acidic & alkaline solutions.
Application
Triton™ X-114 has been used:
To remove endotoxin from solutions of protein using phase separation technique
In the extraction and purification of DNA from endotoxin
In the biphasic extraction of lipoproteins from bacterial cell wall
As a non-ionic detergent with a low cloud point (23 °C) enabling protein solubilization with phase-partitioning of hydrophilic from amphiphilic proteins

Legal Information
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow