General descriptionPOU domain, class 4, transcription factor 1 (UniProt: Q01851; also known as Brain-specific homeobox/POU domain protein 3A, Brain-3A, Brn-3A, Homeobox/POU domain protein RDC-1, Oct-T1) is encoded by the POU4F1 (also known as BRN3A, RDC1) gene (Gene ID: 5457) in human. Brn-3A is a multifunctional transcription factor with different regions mediating its different effects. It regulates the expression of specific genes involved in differentiation and survival within a subset of neuronal lineages. It is expressed in the brain and the retina and is present in the developing brain, spinal cord and eye. Its expression peaks early in embryogenesis and is undetectable 14 days after birth. Its C-terminal domain can act as both DNA-binding domain and a transcriptional activator. The N-terminal domain is also required for transactivation activity on some target genes acting as a discrete activation domain. Brn-3A acts by binding to sequences related to the consensus octamer motif 5′-ATGCAAAT-3′ in the regulatory regions of its target genes. The POU-specific domain of Brn-3A is localized to amino acids 261-338. Brn-3A is shown to activate Bcl-2 expression and protect neuronal cells from apoptosis. Two isoforms of Brn-3A have been described that are produced by alternative splicing. Isoform 2 can act as a transcription factor but cannot regulate the expression of the same subset of genes and does not have any anti-apoptotic effect on neuronal cells. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency".
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ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.ImmunogenKLH-conjugted linear peptide corresponding to 19 amino acids from the internal region of human homeobox/POU domain protein 3A (Brn-3a).
ApplicationAnti-Brn3a, clone 4L5 ZooMAb , Cat. No. ZRB1098, is a highly specific rabbit recombinant monoclonal antibody antibody that targets Brn-3a and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: 1:1,000-1:10,000 from a representative lot detected Brn3a in mouse and rat brain tissue lysates
Immunocytochemistry Analysis: A 1:40 dilution from a representative lot detected Brn3a in Neuro2A cell line.
Immunohistochemistry (Paraffin) Analysis: A 1:10,000 dilution from a representative lot detected Brn3a in human and mouse retina tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol ActionsClone 4L5 ZooMAb is a rabbit recombinant monoclonal antibody that specifically detects Brain-specific homeobox/POU domain protein 3A, (Brn-3a).
Physical formPurified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation NoteRecommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our
reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal InformationZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
