General description
Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 (UniProt: Q92835; also known as EC:3.1.3.86, Inositol polyphosphate-5-phosphatase D, Inositol polyphosphate-5-phosphatase of 145 kDa, SIP-145, Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, SH2 domain-containing inositol 5′-phosphatase 1, SH2 domain-containing inositol phosphatase 1, SHIP-1, p150Ship, hp51CN) is encoded by the INPP5D (also known as SHIP, SHIP1) gene (Gene ID: 3635) in human. SHIP-1 is a phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes PtdIns(3,4,5)P3 to produce PtdIns(3,4)P2, thereby negatively regulates the PI3K pathways. Its expression is observed in immune and hematopoietic cells. It is detected in about 74% of immature CD34+ cells. It is activated upon translocation to the sites of synthesis of PtdIns(3,4,5)P3 in the membrane. SHIP-1 acts as a negative regulator of B-cell antigen receptor signaling. It also serves as a negative regulator of myeloid cell proliferation/survival and chemotaxis, mast cell degranulation, immune cells homeostasis, integrin IIb/β3 signaling in platelets and JNK signaling in B-cells. Loss of SHIP1 activity is reported to cause myeloid lung consolidation and severe inflammation in the ileum. Its transient inhibition in cancer cell can lead to caspase-8 activation and apoptosis. Human SHIP-1 contains an N-terminal SH2 domain (aa 5-101) that mediates interactions with a large number of intracellular signaling proteins. It has three SH3-interacting motifs (aa 124-129, 969-974, 1040-1051) and two NPXY sequence motifs (aa 912-915 and 1019-1022) that are found in many tyrosine-phosphorylated proteins and are required for the specific binding of the PID domain. Three isoforms of SHIP-2 have been described that are produced by alternative splicing. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Fernandez, S., et al. (2013). Ann. N Y Acad. Sci,1280(1); 6-10; Kavanaugh, WM., et al. (1996). Curr. Biol. 6(4); 438-445).
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ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 22 amino acids from the C-terminal half of human SH2 domain-containing inositol 5′-phosphatase 1 (SHIP1).
Application
Quality Control Testing
Evaluated by Western Blotting in Daudi cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SHIP1 in Daudi cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected SHIP1 in Ramos cell lysate.
Affinity Binding Assay: A representative lot of this antibody bound SHIP1 peptide with a KD of 3.8 x 10-8 in an affinity binding assay.
Flow Cytometry Analysis: 0.1 µg from a representative lot detected SHIP1 in one million THP-1 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected SHIP1 in Human tonsil tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected SHIP1 in Ramos cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 1J20 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detect SH2 domain-containing inositol 5′-phosphatase 1 (SHIP1). It targets an epitope within 22 amino acids within the C-terminal half.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
