| Quantity | 100 μL |
|---|---|
| Volume (Metric) | 100μL |
| Product Line | eBioscience |
| Product Type | Cell Stimulation Cocktail |
| Unit Size | Each |
Answer:Phorbol 12-myristate 13-acetate (PMA) and ionomycin.
Answer:A 500X concentrated cocktail supplied in 100 µL.
Answer:Cell activation and cytokine induction for functional assays, immunoassays and intracellular cytokine flow cytometry.
The Invitrogen eBioscience Cell Stimulation Cocktail is a 500X reagent containing phorbol 12-myristate 13-acetate (PMA) and ionomycin for activation of responsive cultured cells. PMA and ionomycin stimulate cytokine production, enabling downstream functional assays, intracellular cytokine staining and flow cytometry workflows. The product is supplied as 100 µL of concentrated cocktail and is diluted to the working strength before addition to cell cultures. It can be combined with an appropriate protein transport inhibitor when intracellular accumulation of induced cytokines is required for subsequent analysis.
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