Thermo Fisher, 00-5123-43, eBioscience™ Fixation/Permeabilization Concentrate
Quantity: 30 mL, Unit Size: Each
Catalog Number: 00-5123-43
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Category
Pack Size30 mL
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| Includes | One 30 mL bottle |
|---|---|
| Quantity | 30 mL |
| Reagent Type | Fixation Reagent, Permeabilization Reagent |
| Product Line | eBioscience |
| Product Type | Fixation/Permeabilization Buffer |
| Unit Size | Each |
Answer:It is supplied as a 4X concentrate in a 30 mL bottle.
Answer:The matching eBioscience Fixation/Permeabilization Diluent is used to prepare the working solution.
Answer:It supports intracellular flow cytometry staining of targets such as Foxp3, Ki-67, and other nuclear or intracellular proteins.
Invitrogen eBioscience Fixation/Permeabilization Concentrate is a 4X reagent used to prepare the working fixation and permeabilization solution for intracellular flow cytometry. It is supplied as a 30 mL bottle and is mixed with the compatible Fixation/Permeabilization Diluent before cell treatment. The resulting working solution is used during staining workflows for nuclear transcription factors, including Foxp3 and Ki-67, and other selected intracellular targets. Subsequent permeabilization buffer steps allow antibodies to enter fixed cells during staining. The concentrate is a dedicated formulation within the Foxp3/Transcription Factor Staining Buffer system and is not a standalone antibody, fluorescent label, or complete staining kit. Using the matched diluent and buffer system helps ensure consistent fixation chemistry across flow cytometry experiments.
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