Thermo Fisher, 00-5223-56, eBioscience™ Fixation/Permeabilization Diluent
Quantity: 100 mL, Unit Size: Each
Catalog Number: 00-5223-56
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Category
Pack Size100 mL
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| Quantity | 100 mL |
|---|---|
| Reagent Type | Fixation Diluent, Permeabilization Diluent |
| Product Line | eBioscience |
| Product Type | Fixation/Permeabilization Diluent |
| Unit Size | Each |
Answer:It is used to prepare a working fixation/permeabilization solution for intracellular flow cytometry staining.
Answer:The matching Fixation/Permeabilization Concentrate and Permeabilization Buffer are used in the transcription-factor staining workflow.
Answer:No. It is an individual 100 mL component; the concentrate and permeabilization buffer are separate reagents.
Invitrogen eBioscience Fixation/Permeabilization Diluent is a component of the Foxp3/Transcription Factor Staining Buffer workflow for intracellular flow cytometry. The 100 mL reagent is combined with the corresponding 4X Fixation/Permeabilization Concentrate to prepare a working solution used before intracellular antibody staining. This preparation helps preserve cellular targets while providing access for antibodies against transcription factors and other intracellular proteins, including Foxp3 and Ki-67. A separate Permeabilization Buffer is required for the subsequent washing and staining steps. The diluent is supplied as an individual reagent rather than a complete multi-component staining kit. The protocol should be followed as a coordinated fixation and permeabilization procedure to maintain consistent intracellular signal and minimize background.
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