| Column Format | Analytical Column |
|---|---|
| Column Type | Ion Exchange |
| Flow Rate | 1 mL/min |
| For Use With | ASRS, DX-500 BioLC Chromatography System |
| Green Features | FSC |
| Max. Pressure | 4000 psi (275 bar) |
| Mobile Phase Compatibility | pH 2 to 12.5, 100% compatible with common organic solvents, Ionic form eluents: chloride, acetate, or bromide in lithium, sodium or ammonium forms |
| Packing Material | Spherical, Nonporous Polymer |
| Quantity | Each |
| Stationary Phase | Quaternary Ammonium |
| Temperature | 90°C |
| Diameter (Metric) | 4 mm |
| Length (Metric) | 250 mm |
| Particle Size | 13 μm |
| Phase | Ion Exchange |
| Pore Size | Nonporous |
| Product Line | DNAPac |
| Technique | Bio-LC |
| Type | Analytical Column |
| pH | 2 to 12.5 |
| Unit Size | Each |
Answer:It is 4 mm in internal diameter and 250 mm long.
Answer:It uses a strong anion-exchange stationary phase with quaternary-amine-functionalized MicroBeads.
Answer:It supports pH 2–12.5 and a maximum pressure of 4000 psi (275 bar).
DNAPac™ PA100 Oligonucleotide HPLC Columns is designed for strong anion-exchange HPLC analysis and purification of synthetic and chemically modified oligonucleotides. This exact analytical column is 250 mm long with a 4 mm internal diameter, rather than the shorter guard-column format or wider preparative options in the DNAPac family. Its stationary phase uses 13 µm pellicular nonporous polymer particles bearing quaternary-amine-functionalized MicroBeads. The ion-exchange chemistry separates oligonucleotides by interactions with their phosphate backbone and is capable of resolving full-length products from closely related n–1 species. The column also supports the analysis of phosphorothioate oligonucleotides and molecules that form secondary structures. The recommended flow rate is 1 mL/min; its maximum operating pressure is 4000 psi (275 bar), and the operating pH range is 2 to 12.5. It tolerates common organic solvents and temperatures up to 90 °C. These specifications suit analytical workflows that must discriminate closely related oligonucleotide products while allowing mobile-phase and temperature adjustments during method development. The standard 4 mm bore can be incorporated into compatible Bio-LC chromatography systems for analytical purification and quality assessment. The analytical configuration is supplied as a single column. The ion-exchange phase can be used with chloride, acetate or bromide salt eluents in lithium, sodium or ammonium forms, allowing method developers to adjust elution conditions without changing the support.
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