Thermo Fisher, 046110, Dionex™ CarboPac™ PA10 IC Columns

Dionex™ CarboPac™ PA10 IC Columns is designed for high-performance anion-exchange chromatography of mono- and disaccharides, including carbohydrates obtained from mammalian glycoproteins, food samples and plant materials. This is the 4 mm × 250 mm analytical column, not the shorter guard column within the same PA10 range. The stationary phase consists of 10 µm nonporous polymer beads covered with a fine layer of functionalized latex MicroBead resin. Its pellicular construction allows efficient mass transfer and rapid re-equilibration during carbohydrate analysis. Used with pulsed amperometric detection, the column enables detection without derivatizing the sugars. Its selectivity helps separate fucose from the system void and interfering compounds in protein hydrolysates; the chemistry can also support acidic, neutral and amino monosaccharide analysis and separations of sialic acids when sodium acetate is added to the eluent. The analytical bed has a 100 µeq capacity, a recommended 1.0 mL/min flow rate and a maximum pressure of 4000 psi (275 bar). It tolerates eluents spanning pH 0–14 and up to 90% common HPLC solvents. The 250 mm bed provides the main separation path for isocratic or gradient HPAE-PAD methods. The 10 µm packing is specifically intended for the CarboPac PA10 carbohydrate method. Its broad mobile-phase compatibility also enables separation protocols that incorporate organic solvent when required by a validated workflow.