Thermo Fisher, 046115, Dionex™ CarboPac™ PA10 IC Columns

Name: Dionex™ CarboPac™ PA10 IC Columns
Brand: Thermo Fisher
SKU: 046115

Column Type: Guard Column, Length (Metric): 50 mm, Diameter (Metric): 4 mm, Unit Size: Each 1, For isocratic separations of mono- and disaccharides in foods, drugs, and plants, the Thermo Scientific™ Dionex™ CarboPac™ PA10 IC Columns provide superior selectivity. Combined with pulsed amperometric detection (PAD), this anion-exchange column delivers high-resolution separations. It is an ideal choice for separating mono- and disaccharides in mammalian glycoproteins. The column efficiently resolves

Catalog Number: 046115

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Category

Ion Chromatography Columns

TagsIon Chromatography Columns Chromatography Columns and Cartridges Carbohydrate and Amino-Acid IC Columns

CollectionsThermo Fisher Chromatography Columns & Cartridges (HPLC/UHPLC, GC, IC, SPE)Thermo Fisher Chromatography Columns & Guard Cartridges (HPLC, UHPLC, GC)Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs

Description

Column Type: Guard Column
Length (Metric): 50 mm
Diameter (Metric): 4 mm
Unit Size: Each 1
Dionex CarboPac PA10 Columns consist of 10μm diameter nonporous beads covered with a fine latex of functionalized Thermo Scientific™ Dionex™ MicroBead™ resin. This pellicular resin structure permits excellent mass transfer, resulting in high-resolution chromatography and rapid re-equilibration.Sensitive Analysis of Mono- and Disaccharides
• Ideal for the quantification of acidic, neutral, and aminomonosaccharides
• Well suited for separation of sialic acids when sodium acetate added to the eluent
• Simple isocratic separations
• No derivatization required
• Gradient compatible
• Available in microbore and standard bore formatsAbout the Dionex CarboPac Family of ColumnsThe Dionex CarboPac family offers a selection of ion chromatography (IC) columns, each optimized for a different class of carbohydrates. Combined with PAD, which provides a means to detect carbohydrates without derivatization, these columns support simple, reliable techniques to separate sugar alcohols, mono- and disaccharides, and poly- and oligosaccharides.Dionex CarboPac columns provide high-resolution separations of:
• Glycoprotein oligosaccharides and complex carbohydrates from dietary fiber, including fructans, maltodextrins, and amylopectins
• Monosaccharides, such as those obtained from glycoprotein hydrolysates or plant hydrolysates
• Mono- and disaccharides

Specifications

General

Column Capacity	20 μeq
Flow Rate	1.0 mL/min.
For Use With (Application)	Carbohydrate Analysis
For Use With (Equipment)	HPAE-PAD
Green Features	FSC
Max. Pressure	4000 psi (275 bar)
Particle Size	10 μm
Quantity	Each 1
Solvent Compatibility	pH 0 to 14, Up to 90% of Common HPLC Solvents
Stationary Phase	CarboPac PA10
Column Type	Guard Column
Diameter (Metric)	4 mm
Format	Standard Bore
Length (Metric)	50 mm
Unit Size	Each 1

FAQs

Question: Is this a full-length analytical column?

Answer:No. This is the 4 mm × 50 mm guard column designed to protect a matching PA10 analytical column.

Question: What is the exchange capacity?

Answer:The guard-column capacity is 20 µeq.

Question: Which chromatography method is supported?

Answer:It supports PA10 carbohydrate separations using high-performance anion exchange with pulsed amperometric detection.

Application Note

Dionex™ CarboPac™ PA10 IC Columns is designed for protecting a CarboPac PA10 analytical column during high-performance anion-exchange chromatography of carbohydrates. This exact configuration is a 4 mm × 50 mm guard column, not the 250 mm analytical column in the same family. Its PA10 packing uses 10 µm nonporous polymer beads bearing functionalized latex MicroBeads, providing a stationary-phase chemistry matched to PA10 carbohydrate separation. Installed upstream of the analytical column, the guard intercepts sample contamination and strongly retained material before these reach the longer separation bed. The chemistry is useful in carbohydrate workflows involving mono- and disaccharides from foods, pharmaceuticals, plants and glycoprotein hydrolysates. Combined with pulsed amperometric detection, the PA10 separation system can detect these analytes without chemical derivatization. This guard format has a 20 µeq column capacity, a stated 1.0 mL/min flow rate and a 4000 psi (275 bar) maximum pressure. It is compatible with mobile-phase conditions from pH 0 to 14 and up to 90% of common HPLC solvents. The 50 mm length and matching 4 mm bore allow guard protection without substituting a full-length analytical bed. The matching PA10 stationary phase helps preserve the analytical method's intended selectivity while the guard column carries the contamination burden. Replace the guard when its performance deteriorates instead of treating it as a standalone 250 mm analytical column.

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