Thermo Fisher, 054993, ProPac™ WCX-10 HPLC Columns

ProPac™ WCX-10 HPLC Columns is designed for weak cation-exchange separation of proteins and protein variants. This analytical column measures 250 mm × 4 mm and contains 10 µm spherical, nonporous polymer particles carrying a carboxylate stationary phase. A thin hydrophilic layer on the particle core reduces unwanted secondary interactions, while the weak cation-exchange surface provides pH-controlled selectivity and fast mass transfer. The column is suited to characterization and quality-control analysis of monoclonal antibodies and related protein variants, including species created by deamidation or lysine truncation. It operates at a typical flow rate of 1 mL/min, supports pressures up to 3000 psi (207 bar), temperatures up to 60 °C and mobile phases from pH 2–12. The phase is compatible with up to 80% acetonitrile or acetone and with nonionic and zwitterionic detergents. Its nonporous 10 µm particles support high-resolution Bio-LC separations of charge variants.