Thermo Fisher, 059128, Acclaim™ 120 C18 Columns

Name: Acclaim™ 120 C18 Columns
Brand: Thermo Fisher
SKU: 059128

Length (Metric): 50 mm, Diameter (Metric): 2.1 mm, Particle Size: 3 μm, Unit Size: Each, Improve the separation efficiency in your reversed-phase applications with Thermo Scientific™ Acclaim™ 120 C18 columns. These columns feature an ultrapure silica substrate with extremely low metal content to minimize tailing effects and deliver symmetrical peak shapes. The unique bonding chemistry yields excellent surface coverage to provide highly predictable separations that are unaffected by secondary int

Catalog Number: 059128

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Category

Reversed-Phase HPLC Columns

TagsHPLC Columns Chromatography Columns and Cartridges HPLC Columns and Cartridges

CollectionsThermo Fisher Chromatography Columns & Cartridges (HPLC/UHPLC, GC, IC, SPE)Thermo Fisher Chromatography Columns & Guard Cartridges (HPLC, UHPLC, GC)Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs

Description

Length (Metric): 50 mm
Diameter (Metric): 2.1 mm
Particle Size: 3 μm
Unit Size: Each
High Performance Reversed-Phase Columns for Reproducible Results
• Very high surface coverage and very low metal content together result in columns with excellent efficiencies
• Reproducible column-to-column performance
• Extremely low bleed, fully compatible with MS
• Stable between pH 2 and 8
• Available in 2.2, 3, and 5μm particle sizes and 4.6, 3.0, and 2.1mm diameters, with an average pore diameter of 120Å
• Also available in 2.2μm RSLC format for reliable, fast separationsAbout the Acclaim Family of HPLC ColumnsAchieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.
• Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs
• Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations

Specifications

General

Column Format	Analytical Column
Column Type	Reversed Phase
Endcapped	Yes
For Use With	LC/MS
Green Features	FSC
Max. Pressure	4500 psi (310 bar)
Packing Material	Spherical, Fully Porous, Ultrapure Silica
Quantity	Each
Stationary Phase	C18
Surface Area	300 m²/g
Temperature	60°C
USP Type	L1
Carbon Load	18%
Diameter (Metric)	2.1 mm
Length (Metric)	50 mm
Particle Size	3 μm
Phase	Reversed Phase
Pore Size	120 Å
Product Line	Acclaim
pH	2 to 8
Unit Size	Each

FAQs

Question: What are the dimensions of this Acclaim 120 C18 column?

Answer:It is 50 mm long, 2.1 mm I.D. and packed with 3 µm particles.

Question: What stationary phase and pore size does it use?

Answer:It uses a C18 phase on fully porous ultrapure silica with a 120 Å pore size.

Question: What are the principal operating limits?

Answer:The column supports pH 2–8, temperatures up to 60 °C and pressures up to 310 bar.

Application Note

Acclaim™ 120 C18 Column 059128 is used for reversed-phase HPLC separations that require a compact, narrow-bore analytical format. This version is 50 mm long with a 2.1 mm internal diameter and contains 3 µm spherical, fully porous ultrapure silica. The C18 stationary phase has a 120 Å pore size, 300 m²/g surface area and 18% carbon load, and it is classified as USP L1. The column operates over pH 2–8, supports temperatures up to 60 °C and is rated to 4500 psi (310 bar). The 2.1 mm I.D. helps reduce solvent consumption compared with conventional 4.6 mm columns, while the 50 mm length supports short analytical runs when the method and sample complexity allow it. The combination of high-purity silica, C18 bonding and 3 µm particles makes the column suitable for routine small-molecule reversed-phase work where efficient separations are needed in a low-volume LC format.

Ordering

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