Thermo Fisher, 060266, Acclaim™ 300 C18 HPLC Columns

Acclaim™ 300 C18 HPLC Column, 150 x 4.6 mm, 3 µm is used for high-resolution reversed-phase peptide mapping and protein separations. The stationary phase is C18 bonded to ultrapure, fully porous silica with a wide 300 Å pore structure, giving larger biomolecules better access to the bonded surface than conventional small-pore reversed-phase packings. The 3 µm particles support faster mass transfer and high separation efficiency, while the 150 mm × 4.6 mm analytical format provides a standard-bore option for bio-LC and LC/MS workflows. The packing is endcapped and has a surface area of about 100 m²/g with an 8% carbon load. The column operates over pH 2.5–7.5, supports temperatures up to 60°C and has a maximum pressure of 4500 psi (310 bar). Low bleed and wide-pore C18 selectivity make it well suited to peptide and protein characterization where MS compatibility and reproducible reversed-phase retention are required.