Thermo Fisher, 066843, Acclaim™ Mixed Mode HILIC-1 HPLC Columns

Acclaim™ Mixed Mode HILIC-1 HPLC Columns is designed for separating polar and nonpolar molecules through combined hydrophilic-interaction and reversed-phase selectivity. This exact analytical column measures 150 mm × 4.6 mm and contains 5 µm fully porous, spherical ultrapure silica particles with 120 Å pores. Its stationary phase consists of a hydrophobic alkyl chain bearing a terminal diol group, allowing the method to exploit HILIC retention for polar compounds as well as reversed-phase behavior. The mixed-mode surface is suitable for nonionic ethoxylated surfactants, drug metabolites, lipids and polyethylene glycols. The phase operates from pH 2.5 to 7.5 and up to 50 °C, with a maximum pressure of 400 bar (5800 psi). The packing has a surface area of 300 m²/g. This configuration supports analytical HPLC method development when highly polar compounds are insufficiently retained by conventional reversed-phase columns but a mixed selectivity offers an alternative to a single-mode phase.