Thermo Fisher, 074625, MAbPac™ SCX-10 HPLC Columns

MAbPac™ SCX-10 is a strong cation-exchange HPLC column for high-resolution separation of monoclonal antibodies and charge variants. This analytical configuration measures 4 mm × 250 mm and uses 10 µm nonporous pellicular particles. A hydrophilic surface layer reduces hydrophobic interactions, while the grafted cation-exchange chemistry provides charge-based selectivity that can resolve closely related mAb species. The column is suited to antibody characterization, method development, stability studies and quality-control assessment where reproducible charge-variant profiles are required. Its 250 mm bed length provides the resolving path needed for analytical separations, while matching SCX-10 guard columns can be installed upstream to protect the analytical bed from contamination. Method conditions should be optimized for the individual antibody, buffer system and required resolution.