Thermo Fisher, 080922-1521232, DNAPac™ RP Columns

Name: DNAPac™ RP Columns
Brand: Thermo Fisher
SKU: 080922-1521232

Length (Metric): 150 mm, Diameter (Metric): 21.2 mm, Unit Size: Each, Achieve superior reversed phase oligonucleotide separations using the Thermo Scientific™ DNAPac™ RP HPLC columns. The unique chemistry is designed for analysis of oligonucleotides and double-stranded (ds) DNA/RNA fragments using LC-UV or LC-MS. The column chemistry provides excellent performance under a broad range of pH, temperature, and mobile phase compositions. In addition, the wide pore size of the resin provides excellen

Catalog Number: 080922-1521232

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Category

Reversed-Phase HPLC Columns

TagsHPLC Columns Chromatography Columns and Cartridges HPLC Columns and Cartridges

CollectionsThermo Fisher Chromatography Columns & Cartridges (HPLC/UHPLC, GC, IC, SPE)Thermo Fisher Chromatography Columns & Guard Cartridges (HPLC, UHPLC, GC)Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs

Description

Length (Metric): 150 mm
Diameter (Metric): 21.2 mm
Unit Size: Each
High Performance Reversed-Phase LC ColumnsThe DNAPac RP column is based on spherical wide-pore 4μm polymer resin. The phase is highly stable to extremes of pH (0-14), and temperature (5-110°C), and operates with mobile phases compatible with direct mass spectrometry. The column may be operated in both HPLC and UHPLC instruments, producing high resolution at high throughput for oligonucleotides and resolves both small and very large dsDNA segments. DNAPac RP reversed phase (RP) column is specifically designed for separation of oligonucleotides and DNA/RNA fragments. The stationary phase is fully compatible with mass spectrometry friendly organic solvents and ion-pair reagents such as triethylamine (TEA) and hexylamine (HA). The wide pore size of polymer resin enables efficient separation of large nucleic acids with low carry-over.Columns designed for reversed phase oligonucleotide LC and LC/MS separations
• Designed for ion-pair reversed-phase (IP-RP) separations of both oligonucleotides and double-stranded nucleic acids
• High resolution and high throughput
• Excellent MS compatibility
• Wide operating pH range (0-14) and high temperature stability (up to 110°C)

Specifications

General

Column Format	Preparative column, standard
Column Type	Reversed Phase
Flow Rate	4 to 8 mL/min
Max. Pressure	2900 psi (200 bar)
Packing Material	4 µm polymer Resin
Quantity	Each
Stationary Phase	Proprietary
Temperature	up to 110°C
Diameter (Metric)	21.2 mm
Length (Metric)	150 mm
Material	DVB (Divinylbenzene) Polymer
Particle Size	4 μm
Phase	Reversed Phase
Product Line	DNAPac
Technique	Reversed Phase HPLC
Type	Preparative Column
pH	0 to 14
Unit Size	Each

FAQs

Question: What are the column dimensions?

Answer:This preparative DNAPac RP column is 21.2 mm ID × 150 mm long.

Question: What particle size is used?

Answer:The column is packed with 4 µm polymer resin.

Question: What type of molecules is the column designed to separate?

Answer:It is designed for reversed-phase separation of oligonucleotides and double-stranded DNA or RNA fragments.

Application Note

DNAPac™ RP is a reversed-phase polymeric column designed for high-resolution separation of oligonucleotides and double-stranded DNA or RNA fragments. This configuration is a preparative 21.2 × 150 mm column packed with 4 µm wide-pore divinylbenzene polymer resin. The phase operates across pH 0–14 and temperatures up to 110 °C and is compatible with ion-pair reversed-phase mobile phases used for LC-UV and LC-MS workflows. The large pore structure supports separation of large double-stranded nucleic acids, while the polymeric stationary phase provides broad chemical stability. This preparative format operates at approximately 4–8 mL/min and is rated to 200 bar, supporting larger-load purification or analytical-scale-up workflows.

Ordering

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