Thermo Fisher, 088779, ProPac™ WCX-10 HPLC Columns

ProPac™ WCX-10 HPLC Columns is designed for high-resolution weak cation-exchange separation of proteins and charge variants. The particular analytical column measures 4 mm in internal diameter and 150 mm in length, making it different from the 4 × 250 mm analytical variant and the 4 × 50 mm guard column. Its weak cation-exchange surface provides separation based on charge-related interactions, supporting studies of protein heterogeneity and the analytical characterization of protein variants. The 150 mm bed provides an established format for laboratories developing or transferring liquid-chromatography methods for proteins, including research on monoclonal antibody charge profiles. The WCX-10 phase has carboxylate-type functionality, distinguishing it from strong cation-exchange phases that use sulfonate groups. Recommended operation for the 4 × 150 mm, 10 µm format uses approximately 0.5–1.0 mL/min and a maximum pressure of 3,000 psi. The column is a protein-separation analytical device, not a process-scale purification resin or an ion-chromatography anion column, and it fits workflows that require interpretation of resolved charged species.