Thermo Fisher, 10533065, Terminal Deoxynucleotidyl Transferase, recombinant, 500 U

Quantity: 500 U
Unit Size: Each
Terminal Deoxynucleotidyl Transferase, Recombinant (rTdT) is DNA polymerase that catalyzes addition of deoxynucleotides to 3fi hydroxyl terminus of DNA.
• Source: Purified from baculovirus clone of calf thymus TdT
• Performance and Quality Testing: Endonuclease, 3' and 5' exodeoxyribonuclease, and levels of incorporation tested
• Unit Definition: One unit incorporates 1nmol dATP into acid-precipitable material in 1 hr. at 37°C, using d(pA)_50as primer
• Unit Reaction Conditions: 0.2M potassium cacodylate (pH 7.2), 10mM MgO_4C_4H_6, 1mM 2-mercaptoethanol, 0.5mg/mL BSA, 100 flM d(pA)_50, 1mM [^3H]dATP, and enzyme in 0.15mL for 1 h at 37°CCloning; Restriction Enzyme Cloning; Homopolymer tailing of vector and insert for cloning; Labeling oligonucleotides with biotin, ^32P- or ^35S-label; Apoptosis (TUNEL)