Thermo Fisher, 10902088, Sf-900™ II SFM, 1000 mL

Sf-900™ II SFM, 1000 mL is designed for serum-free, protein-free culture of Spodoptera frugiperda Sf9 and Sf21 insect cells and recombinant-protein production using the baculovirus expression vector system. This ready-to-use liquid medium is supplied as 1,000 mL and supports both suspension and adherent insect cell cultivation. It is suitable for expansion and maintenance of Sf9 or Sf21 cell lines and for the production phase after baculovirus infection. The formulation already contains glutamine, so it does not require routine addition of L-glutamine or surfactants before use. The medium can also support certain other lepidopteran cell lines used in insect-cell expression workflows. Typical culture conditions for Sf9/Sf21 cells include approximately 27–28 °C in vessels with appropriate gas exchange, including shake flasks, spinner bottles and T-flasks. The serum-free, protein-free formulation reduces dependence on serum supplements and helps simplify handling of a recombinant product-containing harvest. The liquid ships at ambient temperature and is stored at 2–8 °C protected from light, with a 12-month shelf life from manufacture.