Description
Quantity: 5 mL
Unit Size: Each
About Dynabeads CD15Dynabeads CD15 are uniform, superparamagnetic polystyrene beads (4.5 μm diameter) coated with a monoclonal mouse IgM antibody recognizing the human CD15 membrane antigen. The CD15 antigen is predominantly expressed on human neutrophil and eosinophil granulocytes, and to a varying degree on monocytes.Magnetic bead-based separation offers easy handlingDynabeads CD15 is added to the sample under continuous mixing to optimize the binding of the Dynabeads to the target cells. By placing the sample on a magnet, the bead-bound target cells are separated from the rest of the sample in just 1 to 2 minutes. For depletion, remove the supernatant to a new tube for further studies and discard the bead-bound cells. For positive isolation for molecular studies, remove the supernatant and wash the bead-bound cells 2 to 3 times in buffer to get optimal purity. The cells can be lysed while still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples can be whole blood, PBMC, or buffy coat.Automation-ready for use with KingFisher purification systemsThe product is ideal for high-throughput workflows using KingFisher systems. A user guide and scripts for KingFisher Flex and Apex workflows are available for download on this page.Learn more about Dynabeads products
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