Thermo Fisher, 11149D, Dynabeads™ CD14

Name: Dynabeads™ CD14
Brand: Thermo Fisher
SKU: 11149D

Quantity: 5 mL, Unit Size: Each, Dynabeads CD14 are superparamagnetic beads coupled with an anti-human CD14 antibody that enable easy isolation or depletion of human CD14^+ cells directly from any sample, including whole blood, bone marrow, buffy coat, peripheral blood mononuclear cells (PBMCs), and tissue digests. Advantages of Dynabeads CD14:, • Fast and efficient isolation of CD14^+cells from any sample—no columns required, • High purity, yield, and viability of positively isolated CD14^+cell

Catalog Number: 11149D

Pricing

Send an RFQ.

View RFQ List

Category

Cell Sorters

Pack Size5 mL

TagsCell Analysis Products Microspheres

CollectionsThermo Fisher Cell Analysis Products: Viability, Apoptosis, ROS & Calcium Imaging Assays Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 5 mL
Unit Size: Each
About Dynabeads CD14Dynabeads CD14 are uniform, superparamagnetic beads (4.5 μm diameter) coated with a primary monoclonal antibody specific for the CD14 membrane antigen mainly expressed mainly on human monocytes. Because of the bead size, Dynabeads CD14 can easily and efficiently isolate or deplete cells from viscous samples such as whole blood and bone marrow in about 30 minutes. Dynabeads CD14 will also afford excellent recovery of high-purity, viable cells for use in downstream molecular studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified. Note that intact cells will not be released from these beads, so if you want to isolate CD14^+ cells for cell-based applications, or you need to check your samples with flow cytometry, check out our total human monocyte product offerings.Magnetic bead to based separation offers easy handlingDynabeads CD14 is added to the sample under continuous mixing to optimize the binding of the Dynabeads to the target cells. By placing the sample on a magnet, the bead-bound target cells are separated from the rest of the sample in just 1 to 2 minutes. For depletion, remove the supernatant to a new tube for further studies and discard the bead-bound cells. For positive isolation for molecular studies, remove the supernatant and wash the bead-bound cells 2 to 3 times in buffer to get optimal purity. The cells can be lysed while still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples can be whole blood, buffy coat, bone marrow, PBMC, or tissue digests.Automation-ready for use with KingFisher purification systemsThe product is ideal for high-throughput workflows using KingFisher systems. A user guide and scripts for KingFisher Flex and Apex workflows are available for download on this page.Learn more about Dynabeads products
• FindDynabeads products for a whole range of applications.
• Findmagnets for Dynabeads separations.

Specifications

General

Cell Type	Monocytes
Includes	5 mL Dynabeads CD14 coated with anti-CD14 monoclonal antibody.
Isolation Technology	Depletion, positive isolation for molecular applications
No. of Cells	Processes ∼2 x 10<sup>9</sup> cells total
Output Viability	>95%
Product Line	DYNAL, Dynabeads
Purity or Quality Grade	Research Grade
Quantity	5 mL
Sample Type	PBMC, Blood
Shipping Condition	Room Temperature
Starting Material Cell No.	1 x 10<sup>7</sup> PBMCs per isolation
Target Species	Human
Diameter (Metric)	4.5 μm
Product Type	Antibody Coated Bead
Unit Size	Each

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:

[email protected]

Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.

Thermo Fisher, 11149D, Dynabeads™ CD14

Send an RFQ.

General

Notice

More from Thermo Fisher.

NaCl (5 M), RNase-free, 100 mL

Potassium Acetate (3 M), pH 5.5, RNase-free

SDS, 20% Solution, RNase-free

RNase A, DNase and protease-free (10 mg/mL)

More from Thermo Fisher.

NaCl (5 M), RNase-free, 100 mL

Potassium Acetate (3 M), pH 5.5, RNase-free

SDS, 20% Solution, RNase-free

RNase A, DNase and protease-free (10 mg/mL)