| For Use With (Equipment) | 7500 System, BioRad iCycler iQ, BioRad iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ |
|---|---|
| No. of Reactions | 500 Reactions |
| Polymerase | Taq DNA Polymerase |
| Product Line | Platinum, SuperScript |
| Product Type | One-Step qRT-PCR Kit |
| Quantity | 500 reactions |
| Sample Type | RNA |
| Shipping Condition | Dry Ice |
| Sufficient For | 500 Reactions |
| Detection Method | Primer-probe |
| For Use With (Application) | Gene Expression |
| PCR Method | 1-step RT-qPCR |
| Reaction Speed | Standard |
| Unit Size | Each |
Answer:This configuration supports 500 one-step qRT-PCR reactions.
Answer:It combines SuperScript III Reverse Transcriptase with Platinum Taq DNA Polymerase.
Answer:Store the components at −20 °C and protect the ROX reference dye from light.
SuperScript™ III Platinum™ One-Step qRT-PCR Kit combines reverse transcription and real-time PCR in a single tube for sensitive detection and quantification of RNA targets. The enzyme mix contains SuperScript III Reverse Transcriptase and Platinum Taq DNA Polymerase, allowing cDNA synthesis and amplification to proceed sequentially without opening the reaction vessel. This 500-reaction configuration is designed for 50 µL reactions and includes 500 µL enzyme mix, 12.5 mL 2X reaction mix, magnesium sulfate, and ROX reference dye. The system supports gene-expression, pathogen, and viral RNA assays using sequence-specific primers and fluorescent probes. It can detect low-copy RNA while maintaining a broad quantitative range. Store the components at −20 °C and protect the ROX dye from light. Reaction setup should be optimized for primer/probe concentration and RNA input.
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