Thermo Fisher, 11995065, DMEM, high glucose, pyruvate, 500 mL

Name: DMEM, high glucose, pyruvate, 500 mL
Brand: Thermo Fisher
SKU: 11995065

Quantity: 500 mL, Unit Size: Each

Catalog Number: 11995065

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Category

Classical Cell Culture Media

Pack Size500 mL

TagsCell Culture Media Supplements and Reagents Classical and Basal Cell Culture Media DMEM Cell Culture Media

CollectionsThermo Fisher Cell Culture Media & Supplements | Serum-Free, Chemically Defined Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 500 mL
Unit Size: Each
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.This DMEM is modified as follows:With: High Glucose, L-glutamine, Phenol Red, Sodium PyruvateWithout: HEPESThe complete formulation is available.Using DMEMDMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO_2 environment to maintain physiological pH.

Specifications

General

Cell Line	HeLa, 293, Cos-7, and PC-12
Cell Type	Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
Concentration	1 X
For Use With (Application)	Mammalian Cell Culture
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Product Line	Gibco
Product Type	DMEM (Dulbecco's Modified Eagle Medium)
Quantity	500 mL
Shelf Life	12 Months From Date of Manufacture
Shipping Condition	Ambient
Classification	Animal Origin-free
Form	Liquid
Serum Level	Standard Serum Supplementation
Sterility	Sterile-filtered
Sterilization Method	Sterile-filtered
With Additives	Glutamine
Without Additives	No HEPES
Unit Size	Each

FAQs

Question: How much glucose does this high-glucose DMEM contain?

Answer:The formulation contains 4.5 g/L D-glucose, equivalent to approximately 25 mM.

Question: Does this DMEM contain pyruvate and L-glutamine?

Answer:Yes. It contains 1 mM sodium pyruvate and 4 mM L-glutamine.

Question: Is this DMEM ready to support all mammalian cell lines without supplementation?

Answer:No. Serum, growth factors or other supplements may be required according to the needs of the chosen cell line.

Application Note

Gibco Dulbecco's Modified Eagle Medium (DMEM), high glucose with sodium pyruvate, is a classical basal medium for many established mammalian cell culture workflows. The formulation contains 4.5 g/L D-glucose, 1 mM sodium pyruvate and 4 mM L-glutamine, together with amino acids, vitamins, inorganic salts and phenol red. Its high-glucose composition provides a nutrient-rich starting medium for compatible cell lines, while sodium pyruvate contributes an additional cellular energy substrate. Cultures normally require supplementation with serum or other growth factors appropriate to the cell type and experimental design. Medium selection also depends on bicarbonate buffering, incubation atmosphere and the metabolic behavior of the cells. This 500 mL format provides liquid basal medium for research cell culture, not a complete defined formulation for every cell line. Maintaining consistent medium composition between passages helps reduce avoidable variability in growth and assay results.

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