Thermo Fisher, 12045076, Hybridoma-SFM, 1000 mL

Hybridoma-SFM is a ready-to-use serum-free, very low-protein medium developed for growth of hybridomas and myeloma cells used in antibody-production workflows. This configuration contains 1000 mL of liquid medium with glutamine and phenol red. The formulation supports both suspension and stationary hybridoma cultures and can also be used with recombinant myeloma lines. Total protein content is below 20 µg/mL, which reduces the amount of exogenous protein carried into downstream antibody purification compared with serum-containing media. No routine supplementation is required for standard use, although cholesterol-dependent lines such as NS0 require additional cholesterol lipid concentrate. The medium is stored at 2–8 °C and protected from light. It is useful for research and production-development workflows where serum-free culture, simplified purification and reproducible medium composition are important. For consistent culture performance, cells should be monitored during adaptation from serum-containing conditions because growth characteristics can change as serum is removed.