Thermo Fisher, 12300067, Hybridoma-SFM, powder

Hybridoma-SFM powder is a dry-format serum-free, very low-protein medium for hybridoma and myeloma culture in antibody-production workflows. This configuration prepares 10 L of medium after reconstitution. The formulation supports suspension and stationary hybridomas as well as compatible recombinant myeloma lines and is designed to promote useful cell densities and IgG production without serum. Once prepared, the medium is complete and does not require routine supplementation; cholesterol-dependent lines such as NS0 require additional cholesterol lipid concentrate. The low protein content, below 20 µg/mL after preparation, can simplify downstream purification by reducing unrelated protein load. The powder format is convenient for laboratories or process-development groups preparing larger batches while retaining control over water quality and preparation volume. Unopened powder is stored at 2–8 °C until use. Preparation should use suitable cell-culture-grade water and controlled mixing so the reconstituted medium reaches the intended composition before cells are introduced.