Thermo Fisher, 12490001, CD CHO AGT™ Medium, 100 L

Name: CD CHO AGT™ Medium, 100 L
Brand: Thermo Fisher
SKU: 12490001

Quantity: 100 L, Unit Size: Each

Catalog Number: 12490001

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Category

Chemically Defined Media

Pack Size100 L

TagsCell Culture Media Supplements and Reagents Cell Target Type Cell Culture Media Chinese Hamster Ovary Cell Culture Media

CollectionsThermo Fisher Cell Culture Media & Supplements | Serum-Free, Chemically Defined Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 100 L
Unit Size: Each
CD CHO AGT™ (Advanced Granulation Technology) medium is a protein-free, chemically-defined medium optimized for the growth of Chinese hamster ovary (CHO) cells and expression of recombinant proteins in suspension culture. The AGT™ dry media format is designed to provide increased consistency and productivity across all stages of production from development to commercial manufacturing. CD CHO AGT™ medium contains no proteins or peptide components of animal, plant, or synthetic origin, as well as no undefined lysates or hydrolysates.When to Use CD CHO Medium
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)When to Consider Another Gibco™ MediumConsiderCD FortiCHO™ mediumwhen:
• The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processingConsiderCD OptiCHO™ mediumwhen:
• You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is finicky, i.e. hard to grow or adaptConsiderCD DG44 mediumwhen:
• You use parental DG44 or DXB11 CHO cells (dhfr-)Liquid Media FormulationsGibco™ AGT™ media are also available in aliquid formatthat offers the same performance and formulation as the AGT™ media format. Gibco™ liquid media are manufactured animal origin-free with the same complete, serum-free, protein-free, chemically-defined formulation and performance as their AGT™ media counterparts.Design Your Own Media Specific to Your NeedsWhether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. WithGibco™ cGMP Media Custom Servicesyou can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.

Specifications

General

Cell Line	CHO
Concentration	1 X
Culture Environment	CO2
Endotoxin Level	Low
Format	Ropak Bucket
Glucose Concentration	6.33 g/L
Included Antibiotics	No antibiotics
Inorganic Salts	Calcium, Magnesium, Sodium Phosphate
Product Line	AGT, Gibco
Product Type	CD CHO Medium
Purity or Quality Grade	Certified
Quantity	100 L
Shipping Condition	Dry Ice
Classification	Serum-free
Culture Type	Suspension Cell Culture
Form	Powder (AGT)
Serum Level	Serum-free
Sterility	Non-sterile
With Additives	Sodium Bicarbonate
Without Additives	No Glutamine
Unit Size	Each

FAQs

Question: Which cells is CD CHO AGT Medium designed for?

Answer:It is formulated for suspension culture of Chinese hamster ovary cells and recombinant-protein expression workflows.

Question: Is the medium chemically defined and serum-free?

Answer:Yes. The medium is chemically defined, protein-free, serum-free and animal-origin-free.

Question: Does the formulation contain L-glutamine?

Answer:No. An appropriate glutamine source such as L-glutamine or GlutaMAX supplement should be added according to the process requirements.

Question: What volume does this package prepare?

Answer:This configuration is supplied for preparation of 100 L of medium.

Question: How should the dry medium be stored?

Answer:Store the dry medium at 2–8°C and protect it from light.

Application Note

CD CHO AGT™ Medium is a chemically defined, protein-free and serum-free medium formulated for suspension culture of Chinese hamster ovary (CHO) cells and recombinant-protein production. The Advanced Granulation Technology (AGT) powder format is designed for direct addition to water and large-volume media preparation, helping simplify preparation compared with conventional dry-powder handling. The formulation is animal-origin-free and does not contain L-glutamine, so an appropriate glutamine source such as L-glutamine or GlutaMAX™ supplement should be added according to the selected process. The medium is supplied as a non-sterile 100 L preparation and is stored at 2–8°C protected from light before use. It is suited to CHO process development, scale-up and production-support workflows requiring a defined medium platform.

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