Thermo Fisher, 12490025, CD CHO AGT™ Medium, 10 L

Name: CD CHO AGT™ Medium, 10 L
Brand: Thermo Fisher
SKU: 12490025

Quantity: 10 L, Unit Size: Each

Catalog Number: 12490025

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Category

Chemically Defined Media

Pack Size10 L

TagsCell Culture Media Supplements and Reagents Cell Target Type Cell Culture Media Chinese Hamster Ovary Cell Culture Media

CollectionsThermo Fisher Cell Culture Media & Supplements | Serum-Free, Chemically Defined Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 10 L
Unit Size: Each
CD CHO AGT™ (Advanced Granulation Technology) medium is a protein-free, chemically-defined medium optimized for the growth of Chinese hamster ovary (CHO) cells and expression of recombinant proteins in suspension culture. The AGT™ dry media format is designed to provide increased consistency and productivity across all stages of production from development to commercial manufacturing. CD CHO AGT™ medium contains no proteins or peptide components of animal, plant, or synthetic origin, as well as no undefined lysates or hydrolysates.When to Use CD CHO Medium
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)When to Consider Another Gibco™ MediumConsiderCD FortiCHO™ mediumwhen:
• The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processingConsiderCD OptiCHO™ mediumwhen:
• You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is finicky, i.e. hard to grow or adaptConsiderCD DG44 mediumwhen:
• You use parental DG44 or DXB11 CHO cells (dhfr-)Liquid Media FormulationsGibco™ AGT™ media are also available in aliquid formatthat offers the same performance and formulation as the AGT™ media format. Gibco™ liquid media are manufactured animal origin-free with the same complete, serum-free, protein-free, chemically-defined formulation and performance as their AGT™ media counterparts.Design Your Own Media Specific to Your NeedsWhether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. WithGibco™ cGMP Media Custom Servicesyou can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.

Specifications

General

Cell Line	CHO
Endotoxin Level	≤10 EU/mL, Low
Format	Securitainer Capped Vial
Included Antibiotics	No antibiotics
Product Line	AGT, Gibco
Product Type	CD CHO Medium
Quantity	10 L
Reconstituted Volume	10 L
Shipping Condition	Wet Ice
Species	Hamster
Classification	Chemically-defined
Culture Type	Suspension Cell Culture
Form	Powder (AGT)
Serum Level	Serum-free
With Additives	Sodium Bicarbonate
Without Additives	No Glutamine
Unit Size	Each

FAQs

Question: What type of cell culture is CD CHO AGT™ Medium used for?

Answer:It is used for suspension culture of CHO cells and recombinant protein expression.

Question: Is the medium chemically defined and animal-origin free?

Answer:Yes. It is chemically defined, serum-free, protein-free and animal-origin free, with no undefined lysates or hydrolysates.

Question: What is the AGT™ format?

Answer:AGT™ is a granular dry-media format that provides a complete formulation with rapid dissolution and simplified scale-up from development to manufacturing.

Application Note

CD CHO AGT™ Medium, 10 L is used to support suspension culture of Chinese hamster ovary cells and recombinant protein expression in serum-free bioprocess workflows. The medium is chemically defined and protein-free, with no protein or peptide components of animal, plant or synthetic origin and no undefined lysates or hydrolysates. Its AGT™ dry granular format provides a complete formulation that is pre-adjusted for pH and osmolality and dissolves rapidly during media preparation. The formulation contains sodium bicarbonate and is supplied without glutamine, phenol red, sodium pyruvate, thymidine or hypoxanthine, allowing the process to add required supplements separately. CD CHO AGT™ Medium supports suspension cell culture from development through scale-up and is suitable for CHO processes in which a defined, animal-origin-free medium is required. This pack prepares 10 L of reconstituted medium and is stored refrigerated at 2–8°C before preparation.

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