Thermo Fisher, 12587001, B-27™ Supplement (50X), minus vitamin A

Name: B-27™ Supplement (50X), minus vitamin A
Brand: Thermo Fisher
SKU: 12587001

Quantity: 100 mL, Unit Size: Each, Gibco B-27 Supplement minus vitamin A is a customized, serum-free formulation based on the original, most cited neuronal cell culture supplement, designed specifically to support the proliferation and cultivation of neural stem cells (NSCs) and neural progenitor cells. By excluding vitamin A (also known as retinyl acetate or retinol), this variant prevents the differentiation-inducing effects of retinoic acid, making it ideal for maintaining NSCs and neural pro

Catalog Number: 12587001

Pricing

Send an RFQ.

View RFQ List

Category

Classical Cell Culture Media

Pack Size100 mL

TagsCell Culture Media Supplements and Reagents Cell Culture Reagents Antibiotics and Supplements Additional Cell Culture Reagents and Supplements

CollectionsThermo Fisher Cell Culture Media & Supplements | Serum-Free, Chemically Defined Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 100 mL
Unit Size: Each
• Optimized for NSC proliferation—by removing vitamin A, this supplement is perfectly suited for the growth and expansion of embryonic and adult neural stem cells and neural precursors, without inducing differentiation
• Versatile cultivation—supports the cultivation of neural progenitor cells and NSCs in both neurospheres in suspension and adherent monolayer cultures, providing flexibility in your experimental design. Ideal for cell culture where retinoic acid signaling must be controlled.
• High quality—cGMP-manufactured to ensure the highest standards of quality and consistency. Each batch undergoes rigorous performance testing, including assessments of its ability to support the differentiation of human neural stem cells to neurons.
• Comprehensive testing—every lot is tested for endotoxins, sterility, and mycoplasma to ensure the utmost safety and reliability
• Proven performance—derived from the proprietary formulation of the original B-27 Supplement, trusted by researchers worldwide for its consistent and reliable resultsChoose Gibco B-27 Supplement, minus vitamin A, for an optimized, high-quality solution that supports the proliferation of neural stem cells while preventing unwanted differentiation influenced by retinoic acid, ensuring consistent and reliable results for your research.

Specifications

General

Cell Type	Neural Progenitor Cells
Concentration	50 X
Culture Type	Mammalian Cell Culture
Product Line	B-27
Quantity	100 mL
Serum Level	Serum-Free
Shelf Life	12 Months
Shipping Condition	Dry Ice
Source	Serum-Free
Tested For	Endotoxin, Performance, Sterility
Classification	Serum-free
Form	Liquid
Product Type	Cell Culture Supplement
Sterility	Sterile
Unit Size	Each

FAQs

Question: What distinguishes this B-27 supplement from the original formulation?

Answer:It omits vitamin A to support neural stem and progenitor cell proliferation where differentiation should be limited.

Question: What size and concentration are supplied?

Answer:It is a 100 mL package of 50X liquid supplement.

Question: How should it be stored?

Answer:Store at −5 to −20°C and protect from light.

Application Note

B-27™ Supplement (50X), minus vitamin A is designed to support proliferation and culture of neural stem cells and neural progenitor cells while avoiding the differentiation-promoting influence of added vitamin A. This serum-free liquid formulation omits vitamin A, including the retinyl acetate component associated with the standard B-27 formulation. It is particularly suited to experiments that maintain neural precursors in an undifferentiated state or investigate conditions in which retinoic-acid signaling must be controlled. The supplement can be used with suitable Neurobasal or Neurobasal-A basal media for neurosphere suspension culture or adherent monolayer approaches. This configuration is a 100 mL package at 50X concentration, providing a larger supply than the separate 10 mL option while retaining the same minus-vitamin-A formulation. The product is sterile, serum-free, performance-tested and shipped frozen. Store at −5 to −20°C protected from light, and thaw at refrigerated or room temperature rather than in a heated water bath. It is a culture supplement, not a complete basal medium on its own.

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:

[email protected]

Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.