Thermo Fisher, 12594100, SuperScript™ IV One-Step RT-PCR System

Name: SuperScript™ IV One-Step RT-PCR System
Brand: Thermo Fisher
SKU: 12594100

Includes: Kit only, No. of Reactions: 100 Reactions, Unit Size: Each, The Invitrogen SuperScript One-Step RT-PCR System, available with or without ezDNase, combines the high processivity of SuperScript IV Reverse Transcriptase (RT) with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance. With this convenient one-step formulation plus gene-specific primers, you can perform both cDNA synthesis from total RNA or mRNA and PCR amplification in a sing

Catalog Number: 12594100

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Category

One-Step RT-PCR Kits

Pack Size1 ea / pack

TagsPCR Equipment and Supplies PCR Supplies Reverse Transcription Products One-Step Reverse Transcription PCR Reagents

CollectionsThermo Fisher PCR Equipment & Supplies: Instruments, Master Mixes & Plastics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Includes: Kit only
No. of Reactions: 100 Reactions
Unit Size: Each
Features of the SuperScript IV One-Step RT-PCR System include:
• Superior performance with sensitivity down to 0.01 pg RNA, target length up to 13.8 kb, and the fastest one-step RT-PCR protocol
• Reliable target detection even with suboptimally pure RNA samples
• Two-phase hot-start activation mechanism for high sensitivity, specificity, and room temperature setup
• Fast and easy gDNA removal for superior accuracy and confidence in results (SuperScript IV One-Step RT-PCR System with ezDNase only)SuperScript IV RT is a proprietary MMLV RT mutant with increased processivity, thermostability, and inhibitor resistance, as well as reduced RNase H activity, for sensitive and efficient full-length cDNA synthesis. Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR. The combination of these two engineered enzymes produces enhanced RT-PCR yields and fidelity, as well as amplification of longer templates compared to other one-step RT-PCR kits.The inclusion of ezDNase (SuperScript IV One-Step RT-PCR System with ezDNase only) for use upstream of the RT step further enhances the one-step RT-PCR workflow through an extremely simplified genomic DNA removal step that ensures the highest accuracy and confidence in one-step RT-PCR results.

Specifications

General

Final Product Type	PCR Amplified cDNA
Format	Kit
Hot Start	Built-In Hot Start
Includes	Kit only
No. of Reactions	100 Reactions
Optimal Reaction Temperature	45°C to 60°C
Polymerase	Platinum SuperFi
Quantity	1 Ea.
Reaction Format	Master Mix
Reagent Type	Reverse Transcription
Reverse Transcriptase	SuperScript IV
Ribonuclease H Activity	Reduced
Shipping Condition	Dry Ice
Size (Final Product)	Up to 14 kb
Starting Material	RNA
Technique	1-step RT-PCR
Detection Method	Gel Electrophoresis
GC-Rich PCR Performance	High
PCR Method	1-step RT-PCR
Reaction Speed	10 min.
Unit Size	Each

FAQs

Question: What workflow is this system designed for?

Answer:It is designed for end-point one-step RT-PCR, combining cDNA synthesis from RNA and PCR amplification in one tube.

Question: How many reactions are included and what enzymes are used?

Answer:The kit supports 100 reactions and combines SuperScript IV Reverse Transcriptase with Platinum SuperFi DNA Polymerase.

Question: Does this kit include ezDNase?

Answer:No. This is the kit-only version; the separate version with ezDNase is a different product.

Application Note

SuperScript™ IV One-Step RT-PCR System, 100 Reactions is used for end-point one-step RT-PCR, combining reverse transcription of RNA and PCR amplification in a single reaction tube. The system combines SuperScript IV Reverse Transcriptase with Platinum SuperFi DNA Polymerase and uses gene-specific primers to generate and amplify cDNA from total RNA or mRNA. The kit provides 100 reactions and includes SuperScript IV RT Mix, 2X Platinum SuperFi RT-PCR Master Mix, and nuclease-free water; this version does not include ezDNase. It supports an optimal reverse-transcription temperature range of 45–60 °C, built-in hot-start PCR, reduced RNase H activity, and a 10-minute reaction speed for the reverse-transcription step. The system can detect RNA inputs down to 0.01 pg and supports long targets up to approximately 14 kb. It is designed for research workflows that need sensitive, high-fidelity one-step RT-PCR and can tolerate suboptimally pure RNA samples. Components are stored at –20 °C.

Ordering

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Unless otherwise stated, the minimum order amount is USD 1,000.
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