Thermo Fisher, 12604013, TrypLE™ Express Enzyme (1X), no phenol red, 100 mL

Name: TrypLE™ Express Enzyme (1X), no phenol red, 100 mL
Brand: Thermo Fisher
SKU: 12604013

Quantity: 100 mL, Unit Size: Each

Catalog Number: 12604013

Pricing

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Category

Non-Animal Origin Dissociation Reagents

Pack Size100 mL

TagsCell Culture Media Supplements and Reagents Cell Culture Reagents Antibiotics and Supplements Dissociation Reagents

CollectionsThermo Fisher Cell Culture Media & Supplements | Serum-Free, Chemically Defined Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 100 mL
Unit Size: Each
Cleaves peptide bonds on the C-terminal sides of lysine and arginine, and is a direct replacement for trypsin. Exceptional purity increases specificity and reduces damage to cells that can be caused by other enzymes present in some trypsin extracts. Used for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells.
• Gentle on cells
• Room temperature-stable for 24 months
• Easy to use
• Animal origin-free
• Gentle on cells, purity increases specificity due to the action of a single enzyme and reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts
• Can be directly substituted for trypsin in existing protocols
• Dilution alone inactivates TrypLE Express, avoiding the need for trypsin inhibitors, such as FBSEnergy efficient, less hazardous, sustainable packagingCell Culture, Mammalian Cell Culture

Specifications

General

Cell Type	Mammalian Cells
Chelators	EDTA
Concentration	1 X
Culture Type	Adherent Cell Culture
Osmolality	Check and Record
Product Line	Gibco
Quantity	100 mL
Remarks	Room Temperature Stable
Shelf Life	24 Months
Shipping Condition	Room Temperature
Source	Animal origin-free
Tested For	In vitro Bioassay
Classification	Animal Origin-free
Form	Liquid
Product Type	Cell Culture Dissociation Reagent
Sterility	Sterile-filtered
Without Additives	No Phenol Red
pH	6 to 8
Unit Size	Each

FAQs

Question: What is the volume of this TrypLE Express configuration?

Answer:The bottle contains 100 mL of ready-to-use 1X solution.

Question: Does this formulation contain animal-derived trypsin or phenol red?

Answer:No. It uses a recombinant animal-origin-free protease and contains no phenol red.

Question: What is the main application?

Answer:It is used to dissociate adherent mammalian cells for passaging, harvesting and related cell-culture workflows.

Application Note

TrypLE™ Express Enzyme (1X), no phenol red is used to detach adherent mammalian cells during passaging, harvesting and cell-expansion workflows. The reagent contains a recombinant, animal-origin-free protease that provides trypsin-like cleavage activity without relying on porcine or bovine trypsin preparations. This 100 mL configuration is supplied at 1X concentration, contains EDTA and does not contain phenol red. It can dissociate a wide range of adherent mammalian cells, including CHO and HEK293 cultures, and supports both serum-free and serum-supplemented culture systems. The ready-to-use liquid is sterile-filtered and stable at room temperature when stored under the specified conditions. Its defined composition and convenient handling make it suitable for routine laboratories as well as workflows that seek to reduce animal-derived components in cell-culture processing.

Ordering

Notice

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