Thermo Fisher, 12610010, CD DG44 Medium

Name: CD DG44 Medium
Brand: Thermo Fisher
SKU: 12610010

Quantity: 1 L, Unit Size: Each

Catalog Number: 12610010

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Category

TagsCell Culture Media Supplements and Reagents Classical and Basal Cell Culture Media Additional Selective and Differential Cell Culture Media

CollectionsThermo Fisher Cell Culture Media & Supplements | Serum-Free, Chemically Defined Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 1 L
Unit Size: Each
CD DG44 Medium is a chemically defined, protein-free medium optimized for the growth of dihydrofolate reductase deficient (DHFR-) Chinese Hamster Ovary (CHO) cells and expression of recombinant proteins in suspension culture. CD DG44 fully chemically defined formulation contains no lysates, hydrolysates, nor proteins or peptide components of animal, plant, or synthetic origin. When to Use CD DG44 Medium
• You use parental DG44 or DXB11 CHO cells (dhfr-)When to Consider Another Gibco™ MediumConsiderCD FortiCHO™ mediumwhen:
• The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processingConsiderCD OptiCHO™ mediumwhen:
• You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is finicky, i.e. hard to grow or adaptConsiderCD CHO mediumwhen:
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)Design Your Own Media Specific to Your NeedsWhether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. WithGibco™ cGMP Media Custom Servicesyou can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.

Specifications

General

Cell Line	CHO DG44
Concentration	1 X
Endotoxin Level	Low
Format	Bottle(s)
Included Antibiotics	No antibiotics
Product Line	Gibco
Product Type	CD DG44 Medium
Quantity	1 L
Shipping Condition	Room Temperature
Classification	Chemically-defined
Culture Type	Suspension Cell Culture
Form	Liquid
Serum Level	Serum-free
With Additives	Sodium Pyruvate
Without Additives	No Glutamine
Unit Size	Each

FAQs

Question: Which cell lines are targeted by this medium?

Answer:It is optimized for DHFR-deficient DG44 and DXB11 CHO cells.

Question: Does the medium contain protein or hydrolysates?

Answer:No. It is chemically defined and contains no proteins, peptides, lysates or hydrolysates.

Question: What volume is supplied?

Answer:The package contains 1 L of liquid medium.

Application Note

CD DG44 Medium is designed for serum-free, chemically defined suspension culture of dihydrofolate-reductase-deficient Chinese hamster ovary cells and recombinant-protein production. This 1 L Gibco CD DG44 liquid medium is optimized for DG44 and DXB11 CHO cell lines carrying the DHFR-deficient phenotype. Its fully chemically defined formulation excludes lysates, hydrolysates, proteins and peptides of animal, plant or synthetic origin, helping reduce the complexity and variability of culture-derived raw materials. It is suitable for growth of parental DHFR-negative CHO cells and expression of recombinant proteins in suspension culture. The formulation provides a defined medium platform for cell-line cultivation and expression workflows that have been developed around DG44 or DXB11 host cells. Compared with undefined serum-containing media, the absence of hydrolysates and proteinaceous components helps simplify characterization of the medium input during upstream bioprocess development. It is supplied as a ready-to-use 1 L liquid medium. Its cell-line-specific formulation is important: it should not be treated as interchangeable with media optimized for CHO-K1, glutamine-synthetase-based CHO, or CHO-S cell lines.

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